Additional Selective and Differential Cell Culture Media
NEOGEN LB Broth, Lennox, 500 g
LB Broth, Lennox is used in molecular genetic studies in a laboratory setting. LB Broth, Lennox is not intended for use in the diagnosis of disease or other conditions in humans.LB Broth formula is based on L Broth described by Lennox for growth and maintenance of E. coli strains used in molecular microbiology procedures. LB Broth is nutritionally rich, formulated for the isolation of pure recombinant strains. E. coli is grown to late log phase in LB Broth. Some plasmid vectors may replicate to high copy numbers without selective amplification. Some vectors may require selective amplification to reach high copy numbers. Chloramphenicol can be added to inhibit host synthesis and, as a result, prevent replication of the bacterial chromosome.For more information visit https://www.neogen.com/solutions/Microbiology/lb-broth-lennox
NEOGEN Harlequin Chromogenic Agar for Salmonella Esterase (CASE), 500 g
CASE is a selective chromogenic agar for the detection of Salmonella that can be used as part of the ISO 6579-1:2017 workflow. It is not intended for use in the diagnosis of disease or other conditions in humans.CASE utilizes a dual chromogenic system to differentiate between Salmonella and non-target organisms that grow on the agar. The first chromogen is a target for esterase activity present in all Salmonella species, and results in blue/green colonies. The second chromogen is a target for ß-glucosidase activity, present in non-target organisms that are able to grow on the agar and also possess esterase activity. Utilization of the second chromogen masks the presentation of the first, resulting in black colonies in non-Salmonella organisms. All other organisms are either inhibited or grow colorless on the agar.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-chromogenic-agar-salmonella-esterase
NEOGEN Agar, Bacteriological No. 1, 500 g
Agar, Bacteriological No. 1 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Agar, Bacteriological No. 1 conforms with the testing specified in the USP/EP Pharmacopeia.Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-bacteriological-number-1
NEOGEN Glucose (Dextrose), 500 g
Glucose is a fermentable carbohydrate and is used when preparing microbiological culture media in a laboratory setting. Glucose is not intended for use in the diagnosis of disease or other conditions in humans.Glucose is a fermentable carbohydrate which acts as an energy source in culture media.For more information visit https://www.neogen.com/solutions/Microbiology/glucose-dextrose
NEOGEN Harlequin Sorbitol MacConkey Agar (SMAC), 500 g
Sorbitol MacConkey Agar (SMAC) is for the isolation of Escherichia coli O157:H7 and is not intended for use in the diagnosis of disease or other conditions in humans. Pathogenicity of Escherichia coli O157:H7, the primary serovar associated with haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS), is linked to the production of verocytotoxins (VT1 and VT2). However, it should be noted that not all strains of O157 produce verocytotoxins, and that strains from other serovars can be toxin producers (e.g. O26, O103, O111, O113, O145). E. coli O157 has been associated epidemiologically with food poisoning outbreaks involving beef burgers and cold cooked meats. This medium is a modification of Sorbitol MacConkey Agar (SMAC).For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-sorbitol-macconkey-agar
NEOGEN Tryptone Glucose Extract (TGE) Agar, 500 g
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-glucose-extract-tge-agar
NEOGEN Yeast Extract Dextrose Chloramphenicol Agar, 500 g
Yeast Extract Dextrose Chloramphenicol Agar is a selective medium for the enumeration of yeasts and molds in milk and other dairy products, and is not intended for use in the diagnosis of disease or other conditions in humans. The medium is said to have superior storage properties to O.G.Y.E. and also has the advantage of incorporating an autoclavable supplement.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract-dextrose-chloramphenicol-agar
NEOGEN M-Green Yeast and Fungi Broth, 500 g
M-Green Yeast and Fungi Broth is used for the detection of fungi in beverages in a laboratory setting. M-Green Yeast and Fungi Broth is not intended for use in the diagnosis of disease or other conditions in humans.M-Green Yeast and Fungi Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast. This formulation is rich in nutrients which allows for excellent fungal growth. Bacterial growth is inhibited by an acid pH.Fungi have been found in potable water and on the inner surface of distribution system pipes. They can survive water treatment or they enter the system after treatment and remain viable.For more information visit https://www.neogen.com/solutions/Microbiology/m-green-yeast-and-fungi-broth
NEOGEN Fastidious Anaerobe Broth, 500 g
Fastidious Anaerobe Agar is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.A primary isolation medium capable of growing most clinically significant anaerobes. Developed by Lab M (Neogen Corporation), comparisons have shown this medium to be superior to other formulations as a primary isolation medium for fastidious organisms. The peptones included have been chosen for maximum growth stimulation. Starch and sodium bicarbonate act as de-toxification agents while hemin encourages pigment production in Porphyromonas melaninogenicus. Specific growth promoting agents are Cysteine for Fusobacterium necrophorum, Propionibacterium acne and Bacteriodes fragilis, arginine for Eubacterium spp. soluble pyrophosphate for Porph. gingivalis and Porph. asaccharolyticus.For more information visit https://www.neogen.com/solutions/Microbiology/fastidious-anaerobe-broth
NEOGEN Columbia CNA Agar, 500 g
Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.For more information visit https://www.neogen.com/solutions/Microbiology/columbia-cna-agar
NEOGEN MRS Agar, 500 g
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp. from various sources and is intended as a substitute for Tomato Juice Agar. This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998. Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.For more information visit https://www.neogen.com/solutions/Microbiology/mrs-agar
NEOGEN WL Nutrient Agar, 500 g
W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans. W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.For more information visit https://www.neogen.com/solutions/Microbiology/wl-nutrient-agar
NEOGEN Tetrathionate (TT) Broth Base, Hajna, 500 g
Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-tt-broth-base-hajna
NEOGEN Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti, 500 g
Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Lithium chloride in the base medium and supplementary antimicrobial compounds Ceftazidime, Polymyxin, Nalidixic acid and Cycloheximide provide the medium’s selectivity. Chromogenic activity is as a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and to a few strains of Enterococci and Bacilli.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-listeria-chromogenic-agar-ottaviani-and-agosti
NEOGEN Eosin Methylene Blue (EMB) Agar Levine, 500 g
Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.This medium was introduced in 1916 by Holt-Harris and Teague to differentiate Escherichia spp. and Aerobacter spp. It was modified by Levine in 1918 who removed sucrose from the formula and increased the lactose content. The distinctive metallic sheen produced by E. coli on this medium is due to acid production resulting in an amide bonding between the eosin and methylene blue, other coliforms do not produce enough acid to cause this reaction. Eosin inhibits most Gram-positive organisms. The prepared medium is sensitive to light.For more information visit https://www.neogen.com/solutions/Microbiology/eosin-methylene-blue-agar-levine